Journal: Acta Veterinaria Scandinavica
Article Title: Gastrointestinal stromal tumour and hypoglycemia in a Fjord pony: Case report
doi: 10.1186/1751-0147-50-9
Figure Lengend Snippet: Both the tumour associated with the stomach and the nodules in the heart showed strong and diffuse positive staining for smooth muscle actin (mouse monoclonal antihuman smooth muscle actin visualized by staining with AEC (3-amino-9ethylcarbazole) labelled secondary antibody, Dako Cytomation, Glostrup, Denmark), prompting a diagnosis of a leiomyosarcoma. Here, positive neoplastic cells (red) are infiltrating (from the left to the right side) in between the negative myocardial cells. Note that also the smooth muscle cells of vessels show strong positive staining (as expected). (anti-smooth muscle actin and HE, 100×).
Article Snippet: The visualization of the antibody-antigen complex was obtained, after rinsing with TBS, by incubation with horseradish peroxidase-labelled polymer conjugated to goat anti-mouse (vimentin, cytokeratin, desmin, SMA) or goat anti-rabbit (CD117) IgG (kits from Dako Cytomation, Glostrup, Denmark) and a final incubation with ready-to-use AEC+ (3-amino-9ethylcarbazole) substrate-chromogen solution for 10 (SMA), 15 (cytokeratin, vimentin and desmin) or 20 (CD117) minutes. (kits from Dako Cytomation, Glostrup, Denmark).
Techniques: Staining